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Tissue Solutions
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SPECTRO Analytical
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Image Search Results
Journal: Nature Communications
Article Title: Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues
doi: 10.1038/s41467-021-27781-9
Figure Lengend Snippet: Protein lysate from a representative male mouse cortex and cerebellum with human plasma as a positive control was treated with or without PNGase F and visualized using biotinylated lectins (ConA, GNL, PHA-E, AAL, RCA, and SNA) in addition to immunoblotting for actin and staining for total protein. Non-specific binding of lectins to PNGase F is noted by an asterisk (*) near 35 kDa, as shown in the Total Protein stain. Protein blotting of brain lysate with each lectin has been repeated at least three times each with similar results. A schematic with common lectin-binding sites is shown for reference. Source data are provided as a Source Data file.
Article Snippet:
Techniques: Clinical Proteomics, Positive Control, Western Blot, Staining, Binding Assay